Australia's Women's Sevens will be without Teagan Levi until at least the final game of day three after their defeat to Great Britain.
Levi was sent off in the fifth minute for a dangerous tackle on Emma Uren, with the head-on-head contact seeing her issued a red card.
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She will join sister Maddison on the sidelines with a three match ban, with the older Levi set to return for the quarter-finals whilst Teagan will need Australia to make the bronze or gold medal match to play again in Perth.
"We have been working on getting our tackle height down but I guess for Teags, she was going in with some really good intent," captain Charlotte Caslick said after the defeat.
"I would never discourage her from the intent she has. It is just the way they are policing the tackle laws now, we have to make sure we are dipping into contact, which has got us into a bit of trouble at the last two tournaments.
"It might be a record, three tournaments, three red cards."
Great Britain had levelled at 7-7 just before Levi was dismissed and, within a minute of the red card, Australia hit the front again after the outstanding Bienne Terita followed her double against South Africa with another five-pointer to give the home team a 12-7 halftime lead.
Emma Uren levelled with a try and GB didn't have to wait long for their decisive score, with Jasmine Joyce racing away.
"It's pretty heartbreaking," Terita said.
"Especially as were excited to play in front of a home crowd, that's not something we want to put out in front of our fans and families and friends, so hopefully we can recover and come back tomorrow better."
Britain leads the pool by two points going into the final round of matches on Saturday, with Australia second ahead of Canada, who they face in their crucial third match.
As for the Men, they enter day two on a high after their comeback victory over GB.
It was one-way traffic in the second half with Henry Palmer. Nathan Lawson and Ben Dowling all crossing as Australia's control of possession and slick passing and finishing proved decisive.
"It wasn't perfect by any measure, we started slow and we can't afford that," Australian playmaker Maurice Longbottom told Stan Sport.
"But we stuck In there and bit down on the mouthguard."
Second-placed Australia are now two points behind the USA and one ahead of Ireland who they play on Saturday
