The Wallabies will be without centre Hunter Paisami for next week's Test against England.
Paisami was originally set to return to Australia at some stage of the tour as he awaits the birth of his child.
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However, the side confirmed a calf injury has ruled the centre out, not travelling to the UK.
"Hunter Paisami has suffered a calf injury and hasn't travelled with the squad for the Spring Tour," a statement read.
"Any additions to the group will be communicated in due course."
His injury opens the door for Samu Kerevi to slot straight back into the starting side after re-joining the squad for the Spring Tour.
New squad member Joseph-Aukuso Suaalii remains an option as he presses for a debut.
2024 debutant Hamish Stewart and Brumbies recruit David Feliuai are in the Australia XV squad in England and may be called into the squad as replacements.
It comes after prop Tom Robertson (calf) was ruled out earlier in the week, pushing Zane Nonggorr into the squad and Tiaan Tauakipulu to the Australia XV squad.
The Wallabies have settled into London before their opening Test against England.
"We're incredibly excited. It's been a couple of years since we've played some of these teams," scrumhalf Tate McDermott said from London.
"Particularly, what a brilliant way to kick off our tour against England at Twickenham/Allianz Stadium.
"We know we're going to have to continue to improve and be ahead of the curve, but we're looking forward to touching down and playing some good footy over the next couple of weeks."
