Maro Itoje has been rested for England's upcoming matches, with Jamie George named as captain for the Tests against South Africa, Fiji and Argentina.
Head coach Steve Borthwick has stood Itoje down from the opening phase of the Nations Championship after second rows Alex Coles and George Martin came through the weekend's action without any fitness issues.
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It follows a year in which Itoje led the British and Irish Lions to a series victory against Australia, played in the pre-Christmas and Six Nations campaigns and mourned the death of his mother.
It would also be in recognition of the 31-year-old having completed more Test minutes than any other player in the game over the last decade.
Borthwick has placed George in charge, with the veteran Lions hooker seen as an ideal alternative given he skippered England throughout 2024 before being replaced by Itoje.
South African-born centre Benhard Janse van Rensburg has been included in Borthwick's 36-man squad having made his England debut in the uncapped game against France on Friday night.
The 29-year-old Bristol centre does not qualify on residency grounds until July 8, meaning he will miss the tour opener against the Springboks in Johannesburg on Saturday week and must wait until Fiji on July 11 for his first Test appearance.
Noah Caluori is rewarded for his breakthrough season at Saracens in which he finished as the Prem's leading try-scorer by also being included among five uncapped players.
Number eight Greg Fisilau and props George Kloska and Vilikesa Sela complete the quintet of Test rookies on a 25,000-mile trip that spans three continents.
"We've selected a squad with a good blend of experience and emerging talent," Borthwick said.
"I am sure by preparing well and continuing to demand the highest standards from one another every day, we will be well placed to meet the challenge of a very demanding summer schedule."
Forwards: Ollie Chessum, Arthur Clark, Alex Coles, Luke Cowan-Dickie, Tom Curry, Theo Dan, Ben Earl, Charlie Ewels, Greg Fisilau, Ellis Genge, Jamie George (capt), Joe Heyes, Ted Hill, George Kloska, George Martin, Beno Obano, Asher Opoku-Fordjour, Guy Pepper, Henry Pollock, Vilikesa Sela
Backs: Seb Atkinson, Noah Caluori, Immanuel Feyi-Waboso, George Ford, Tommy Freeman, George Furbank, Benhard Janse van Rensburg, Alex Mitchell, Cadan Murley, Max Ojomoh, Henry Slade, Fin Smith, Marcus Smith, Ben Spencer, Freddie Steward, Jack van Poortvliet
