England's first Six Nations camp began overnight, with just over a month to go until the tournament begins.
Eddie Jones's men were hit with a handful of injuries after last weekend's Premiership rounds, but plenty of hopefuls will still have a chance to impress before the January 18 announcement of the Six Nations squad.
Charlie Ewels (hamstring), Piers Francis (concussion), Jonathan Joseph (dead leg), Jack Nowell (ankle) and Harry Williams (concussion) were all absent from the camp, while lock Courtney Lawes was already ruled out for personal reasons.
Giant London Irish wing Joe Cokanasiga and Bath's Henry Thomas have been called up as injury replacements.
England stars Maro Itoje and Billy Vunipola have been included in the squad, after lengthy injuries, in a boost to England's Six Nations hopes.
Jones said this two-day camp, in Brighton, was critical.
"This camp in Brighton is important for us to set the tone as a squad ahead of the Six Nations," he said.
"We will need to improve if we are to hunt down our opponents and beat them.
"This tournament will be exciting and challenging, as we know we will play against some very strong teams.
“Brighton will provide a great base to begin our preparations with players coming away with a clear plan for our opening match of the campaign against Italy.”
England has won the past two Six Nations title, losing just one match in the process, to Ireland in 2017.
England opens its Six Nations campaign on Sunday February 4 against Italy, kicking off at 3pm local, Monday 1am AEDT.
England squad for Brighton camp
Mike Brown (Harlequins)
Danny Care (Harlequins)
Joe Cokanasiga (London Irish)
Nathan Earle (Saracens)
Owen Farrell (Saracens)
George Ford (Leicester Tigers)
Jonny May (Leicester Tigers)
Henry Slade (Exeter Chiefs)
Marcus Smith (Harlequins) *
Denny Solomona (Sale Sharks)
Ben Te’o (Worcester Warriors)
Anthony Watson (Bath Rugby)
Ben Youngs (Leicester Tigers)
Dan Cole (Leicester Tigers)
Tom Dunn (Bath Rugby)
Charlie Ewels (Bath Rugby)
Jamie George (Saracens)
Gary Graham (Newcastle Falcons)
Dylan Hartley (Northampton Saints)
James Haskell (Wasps)
Nick Isiekwe (Saracens)
Maro Itoje (Saracens)
Joe Launchbury (Wasps)
Joe Marler (Harlequins)
Zach Mercer (Bath Rugby) *
Beno Obano (Bath Rugby)
Chris Robshaw (Harlequins)
Sam Simmonds (Exeter Chiefs)
Kyle Sinckler (Harlequins)
Henry Thomas (Bath)
Billy Vunipola (Saracens)
Mako Vunipola (Saracens)
Six Nations 2018
Saturday February 3
Wales vs Scotland, Millennium Stadium, 2:15pm local, Sunday 1:15am AEDT
France vs Ireland, Stade de France, 4:45pm local, Sunday 2:45am AEDT
Sunday February 4
Italy vs England, Stadio Olimpico, 3pm local, Monday 1am AEDT
Saturday February 10
Ireland vs Italy, Aviva Stadium, 2:15om local, Sunday 1:15am AEDT
England vs Wales, Twickenham, 4:45pm local, Sunday 3:45am AEDT
Sunday February 11
Scotland vs France, Murrayfield Stadium, 3pm local, Monday 2am AEDT
Friday February 23
France vs Italy, Stade de France, 8pm local, Saturday 6am AEDT
Saturday February 24
Ireland vs Wales, Aviva Stadium, 2:15pm local, Sunday 1:15am AEDT
Scotland vs England, Murrayfield Stadium, 4:45pm local, Sunday 3:45am AEDT
Saturday March 10
Ireland vs Scotland, Aviva Stadium, 2:15pm local, Sunday 1:15am AEDT
France vs England, Stade de France, 4:45pm local, Sunday 3:45am AEDT
Sunday March 11
Wales vs Italy, Millennium Stadium, 3pm local, Monday 2am AEDT
Saturday March 17
Italy vs Scotland, Stadio Olimpico, 12:30pm local, 10:30pm AEDT
England vs Ireland, Twickenham, 2:45pm local, Sunday 1:45am AEDT
Wales vs France, Millennium Stadium, 5pm local, Sunday 4am AEDT