Wallaroos coach Jay Tregonning has called Ashley Marsters and Jemima McCalman into the starting side as he named the team to face the USA.
Marsters slots into the front-row alongside Liz Patu and Bridie O'Gorman after Adiana Talakai suffered a knee injury during Monday's defeat to the Black Ferns.
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This paves the way for Reds hooker Tiarna Molloy to make her debut off the bench, joining teammates Annabelle Codey and Cecilia Smith who earned their first cap last week.
Meanwhile, McCalman comes into the side for her maiden start on the wing, replacing the injured Ivania Wong.
The Brumbies flyer is joined by Waratahs duo Mahalia Murphy and Lori Cramer in the back three.
“Despite the result, we were quite impressed with the team’s performance last week against New Zealand," Tregonning said in a statement.
“We’ve picked up a few bumps and bruises along the way – thankfully we can cover it with the depth we have in this current squad.
“There are a few players that will now get the opportunity to make a mark straight from the kick-off and I know they are extremely eager to make an impact and keep that starting jersey."
The Wallaroos will be hunting for their second win over the USA, who will be eager to bounce back after their defeat to Canada.
“The United States have become a bit of a powerhouse in Women’s Rugby on the world stage and they are going to be a very tough opponent," Tregonning added.
“The only time the Buildcorp Wallaroos have beaten the USA was back in the 2002 Rugby World Cup in Barcelona so a bit of history is on the line for this one.”
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1. Liz Patu – Queensland Reds (26 caps)
2. Ashley Marsters – Melbourne Rebels (13 caps)
3. Bridie O’Gorman – NSW Waratahs (3 caps)
4. Michaela Leonard - Brumbies (7 caps)
5. Kaitlan Leaney – NSW Waratahs (3 caps)
6. Emily Chancellor – NSW Waratahs (9 caps)
7. Shannon Parry (C) - Queensland Reds (15 caps)
8. Grace Hamilton – NSW Waratahs (18 caps)
9. Iliseva Batibasaga – NSW Waratahs (18 caps)
10. Trilleen Pomare – Western Force (14 caps)
11. Jemima McCalman – Brumbies (2 caps)
12. Pauline Piliae-Rasabale – NSW Waratahs (3 caps)
13. Georgina Friedrichs – NSW Waratahs (3 caps)
14. Mahalia Murphy – NSW Waratahs (14 caps)
15. Lori Cramer - NSW Waratahs (7 caps)
Replacements
16. Tiarna Molloy – Queensland Reds*
17. Madison Schuck - Queensland Reds (3 caps)
18. Asoiva (Eva) Karpani – NSW Waratahs (5 caps)
19. Annabelle Codey – Queensland Reds (1 cap)
20. Piper Duck – NSW Waratahs (3 caps)
21. Layne Morgan – NSW Waratahs (3 caps)
22. Cecilia Smith – Queensland Reds (1 cap)
23. Arabella McKenzie – NSW Waratahs (7 caps)
1. Hope Rogers
2. Joanna Kitlinksi
3. Nick James
4. Jordan Matyas
5. Hailie Taufoou
6. Elizabeth Cairns
7. Rachel Johnson
8. Kate Zackary (c)
9. Bridget Kahele
10. Gabby Cantorna
11. Jennine Detiveaux
12. Katana Howard
13. Alev Kelter
14. Lotte Clapp
15. Bulou Mataitoga
Replacements
16. Kathryn Treder
17. Maya Learned
18. Charli Jacoby
19. Kristine Sommer
20. Kathryn Johnson
21. Carly Waters
22. Megan Foster
23. Tess Feury
