England lock George Kruis will not play against Australia, left out of a 25-man squad for the match on Tuesday.
The Saracens second rower has struggled for consistency this season, but was a part of the Lions squad in July to take on the All Blacks.
Kruis was one of 10 players axed from an initial 35-man squad, with the team set to change venues on Wednesday ahead of the Sunday morning (AEDT) clash.
England coach Eddie Jones has instead included Bath's Charlie Ewels in the trimmed-down group, alongside fellow second row options Joe Launchbury, Courtney Lawes and Maro Itoje.
Suggestions that Owen Farrell and Itoje would be rested again seem to have been dispelled, with both players included in the smaller squad on Wednesday morning (AEDT).
Mike Brown, who is under an injury cloud after suffering a concussion against Argentina, was also included.
Prop Joe Marler looks set to come straight back into the team after a suspension, with Leicester's Ellis Genge dropping out of the squad.
England 25-man squad for Australia
Mike Brown (Harlequins), Elliot Daly (Wasps), Jonny May (Leicester Tigers), Semesa Rokoduguni (Bath Rugby), Anthony Watson (Bath Rugby)
Danny Care (Harlequins), Owen Farrell (Saracens), George Ford (Leicester Tigers), Jonathan Joseph (Bath Rugby), Henry Slade (Exeter Chiefs), Ben Youngs (Leicester Tigers)
Charlie Ewels (Bath Rugby), Nathan Hughes (Wasps), Maro Itoje (Saracens), Joe Launchbury (Wasps), Courtney Lawes (Northampton Saints), Chris Robshaw (Harlequins), Sam Simmonds (Exeter Chiefs), Sam Underhill (Bath Rugby).
Dan Cole (Leicester Tigers), Jamie George (Saracens), Dylan Hartley (Northampton Saints), Joe Marler (Harlequins), Mako Vunipola (Saracens), Harry Williams (Exeter Chiefs)
Players released back to their clubs:
Tom Dunn (Bath Rugby)
Piers Francis (Northampton Saints)
Ellis Genge (Leicester Tigers)
Nick Isiekwe (Saracens)
George Kruis (Saracens)
Alex Lozowski (Saracens)
Zach Mercer (Bath Rugby)
Marcus Smith (Harlequins)
Denny Solomona (Sale Sharks)
*Ben Te'o (Worcester Warriors) will remain in camp until Wednesday